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ALGAE > Volume 21(4); 2006 > Article
ALGAE 2006;21(4): 479-483. doi: https://doi.org/10.4490/algae.2006.21.4.479
Ultrastructures of Ptilota filicina (Rhodophyta) by High Pressure Freezing (HPF): Comparison of HPF Fixation and Chemical Fixation
Sang-Hee Lee1*, Youn-Joong Kim1, Jong-Man Jeong1, Jin-Gyu Kim1, Young-Min Kim1, Hee-Seok Kweon1, Won-Jin Moon2 and Seok-Hoon Lee1

1Division of Electron Microscopic Research, Korea Basic Science Institute, Daejeon 305-333, Korea
2KBSI, Gwang-ju Center, Gwangju 500-757, Korea
ABSTRACT
In preparation of the biological samples for electron microscopy, the chemical fixation by glutaraldehyde, paraformaldehyde, and OsO4 has been generally used for a long time. However, the chemical fixation method has some problems: the infiltration time is a little bit long and the ultrastructure of cell or tissue transforms before complete fixation of sample. So, recently, cryo-fixation is considered more often in biomedical field. In this study, we compared High Pressure Freezing (HPF) method with chemical fixation method using a algal sample (Ptilota filicina J. Agardh), which was difficult to fix using chemical fixation method. In chloroplast, the ultrastructure of thylakoid lamella and phycobilisome can not show clearly by chemical fixation. In this study we could observe the ultrastructure of thylakoid lamella and phycobilisome of chloroplast very clearly using HPF fixation. An improved images of ultrastructures of nucleus, mitochondrion and floridean starch could obtain. These results suggest that HPF method is very useful method in algal specimen for electron microscopy.
Key words: freeze substitution, High Pressure Freezing, Ptilota filicina


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