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ALGAE > Volume 28(3); 2013 > Article
ALGAE 2013;28(3): 289-296. doi: https://doi.org/10.4490/algae.2013.28.3.289
Chemiluminescence immunochromatographic analysis for the quantitative determination of algal toxins
Dongjin Pyo1,* and Taehoon Kim1

1Department of Chemistry, Kangwon National University, Chuncheon 200-701, Korea
*Corresponding Author  Email: pyod@kangwon.ac.kr
ABSTRACT
For the quantitative detection of algal toxin, microcystin, a chemiluminescence immunochromatographic assay method was developed. The developed system consists of four parts, chemiluminescence assay strip (nitrocellulose membrane), horse radish peroxidase labeled microcystin monoclonal antibodies, chemiluminescence substrate (luminol and hydrogen peroxide), and luminometer. The performance of the chemiluminescence immunochromatographic assay system was compared with high performance liquid chromatography (HPLC) detection. The detection limit of chemiluminescence immunochromatographic assay system is several orders of magnitude lower than with HPLC. The chemiluminescence immunochromatography and HPLC results correlated very well with the correlation coefficient (r2) of 0.979.
Key words: algal toxin; chemiluminescence immunochromatography; HPLC; microcystin


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