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ALGAE > Volume 7(1); 1992 > Article
ALGAE 1992;7(1): 101-107.
Influence of Light Intensity and Temperature on Callus Development in Young Sporophytes of Four Species of Laminariales (Phaeophyta)
Masahiro Notoya, Mikako Nagashima, Yusho Aruga
Laboratory of Phycology, Tokyo University of Fisheries
ABSTRACT
Excised rectangular blade pieces (0.2-2 mm super(2)) of young sporophytes (3-5 mm in blade length) of four Laminariales species (Costaria costata, Eisenia bicyclis, Laminaria japonica and Undaria pinnatifida) were cultured under various light intensities (1,000-8,000 lux) and temperatures (10-25℃) to observe callus development and differentiation. Callus developed almost in the same manner in each species. After 2-4 days in culture, callus cells were produced from the cut edge of excised blade piece. In 3-4 weeks, blade-like plantlets differentiated from growing callus cells. Favorable conditions for callus development were different with species; 15℃ and 1,000-4,000 lux in C. costata, 20℃ and 1,000 lux in E. bicylclis, 10-15℃ and 1,000 lux in L. japonica, and 15℃ and 2.000 lux in U. pinnatifida.
Key words: callus, development, Laminariales, light intensity, Phaeophyta, temperature, tissue culture, young soprophyte


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