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Algae 1992;7(1): 101-107. |
| Influence of Light Intensity and Temperature on Callus Development in Young Sporophytes of Four Species of Laminariales (Phaeophyta)
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| Masahiro Notoya, Mikako Nagashima, Yusho Aruga
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Laboratory of Phycology, Tokyo University of Fisheries
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| ABSTRACT |
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Excised rectangular blade pieces (0.2-2 mm super(2)) of young sporophytes (3-5 mm in blade length) of four Laminariales species (Costaria costata, Eisenia bicyclis, Laminaria japonica and Undaria pinnatifida) were cultured under various light intensities (1,000-8,000 lux) and temperatures (10-25℃) to observe callus development and differentiation. Callus developed almost in the same manner in each species. After 2-4 days in culture, callus cells were produced from the cut edge of excised blade piece. In 3-4 weeks, blade-like plantlets differentiated from growing callus cells. Favorable conditions for callus development were different with species; 15℃ and 1,000-4,000 lux in C. costata, 20℃ and 1,000 lux in E. bicylclis, 10-15℃ and 1,000 lux in L. japonica, and 15℃ and 2.000 lux in U. pinnatifida.
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| Key words:
callus, development, Laminariales, light intensity, Phaeophyta, temperature, tissue culture, young soprophyte |
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