Effects of Light Intensity and Temperature on Regeneration, Differentiation and Receptacle Formation of Hizikia fusiformis (Harvey) Okamura

Algae > Volume 9(1); 1994 > Article

Algae 1994;9(1): 85-94.

Eun Kyoung Hwang, Chan Sun Park, Chul Hyun Sohn

Department of Aquaculture, National Fisheries University of Pusan

ABSTRACT

The regeneration, differentiation and receptacle formation of Hizikia fusiformis (Harvey) Okamura were investigated to determine the effects of light intensities and temperature for the efficient seed propagation. The vegetative apical portions and holdfasts isolated from naturally grown H. fusiformis were culture under 20 experimental conditions with 5 different levels of light intensity (500-15,000 lux) and 4 different temperatures (5-20℃) under continuous illumination. Regeneration and differentiation occurred in the vegetative apical portions and in the holdfast portions, respectively. Both regeneration and differentiation were enhanced with light intensity of higher than 1,000 lux at 10, 15 and 20℃. Receptacle formation was initiated when thalli reached 9-12 cm in length after 28 days of culture under 15,000 lux at 20℃. Receptacle formation was enhanced with light intensity higher than 1,000 lux at 10, 15 and 20℃, but the embryos were liberated under 5,0,10,000 and 15,000 lux at 10, 15 and 20℃. Both light intensity and temperature affected the subsequent growth rate of regeneration, differentiation as well as receptacle formation.

Key words: Hizikia fusiformis, regeneration, differentiation, receptacle, temperature, light intensity