Influence of Light Intensity and Temperature on Callus Cell Propagation and Differentiation to Bladelets from the Explants of Young Sporophyte of Kjellmaniella crassifolia Miyabe (Phaeophyta, Laminariales)

Algae > Volume 11(1); 1996 > Article

Algae 1996;11(1): 179-182.

Masahiro Notoya, Hyung Geun Kim

Laboratory of Applied Phycology, Tokyo University of Fisheries

ABSTRACT

Excised rectangular blade pieces (0.5 × 0.5 mm) from young sporophytes (3-5 mm in blade length) of Kjellmaniella crassifolia Miyabe were cultured under various light intensities (0-80 μmol m super(-2) s super(-1)) and temperatures (5-25℃) to observe callus cell propagation and differentiation. Favorable culture conditions of callus cell propagation from blade pieces were found to be at 10℃ and 10 μmol m super(-2) s super(-1). However, under conditions of low temperature and low light intensity, callus cells did not differentiate to bladelets. Differentiation of callus cells to bladelets was observed only at a high light intensity of 80 μmol m super(-2) s super(-1). In dark conditions, the callus cells propagated within 16 days, but could not grow after that. Under high light intensities, chloroplasts in the callus cell increased in both size and number, and then the callus cell differentiated to the bladelet. The results suggested that differentiation of the bladelet is related to chloroplasts in the callus cell.

Key words: callus, chloroplast, Kjellmaniella crassifolia, Laminariales, light intensity, Phaeophyta, temperature, tissue culture, young sporophyte