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ALGAE > Volume 13(2); 1998 > Article
ALGAE 1998;13(2): 261-270.
Developmental Morphology on the Regenerationof Pelvetia siliquosa Tseng et Chang (Phaeophyta) in Korea
Jang-Taek Yoon, Woong-Young Soh
National Fisheries Research and Development Agency, Namhae Hatchery, Department of Biology, Chunbuk National University
The morphogenesis of Pelvetia siliquosa was observed from the regeneration of frond segments in PESI media containing different IAA concentration. The healing process of the wounded segments followed the differentiation of the epidermal cells and filamentous cells. The adventitious branches were differentiated from the medullary cells. The regeneration of the frond showed higher activity with 90% at the receptacular fronds, which seemed to be most suitable for regeneration. In apical segments, the adventitious branches developed on the surface of upper fronds after forming conceptacles and releasing eggs and sperms. The development of adventitious branches of the two-year old frond required longer period than those of the one-year old one. The adventitious branches were well developed in lower concentration with 3 mg/l of IAA and were inhibited in higher concentration. The callus were well developed on the surface of upper segments cultured in agar media containing IAA. The process of wound healing, regeneration, development of adventitious branches, and callus formation of P. siliquosa contributed to secure the elementary data for tissue culture of the fucoid algae.
Key words: morphology, Pelvetia siliquosa, Phaeophyta, regeneration

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