The Analysis of Cyanobacterial Neurotoxins by High-Performance Liquid Chromatography-Mass Spectrometry

doi:2003.18.3.233

Algae > Volume 18(3); 2003 > Article

Algae 2003;18(3): 233-238. doi: https://doi.org/10.4490/ALGAE.2003.18.3.233

Jong-Mun Jung, You-Jung Lee, Hong-Ki Park, Eun-Young Jung, Gea-Jea Joo

Department of Biology, Pusan National University, Busan Water Quality Institute, Busan Waterworks HQ

ABSTRACT

Cyanobacteria were dominant from June to September in the Nakdong River and the Hoedong Reservoir. Microcystis aeruginosa was dominant from June to September; Anabaena flos-aquae from June to August and Aphanizomenon flos-aquae from July to August. Cyanobacterial neurotoxins, Anatoxin-a and saxitoxin were analyzed by electrospray ionization-mass spectrometry with strains of Aphanizomenon flos-aquae NIES-81 and Anabaena flos-aquae NIER-10002. Anatoxin-a was not detected from the cultured Anabaena flos-aquae nor from the wild samples. Low levels of saxitoxin were detected in the cultured Aphanizomenon flos-aquae; however, those of field samples were below the detection limit.

Key words: anatoxin-a, Aphanizomenon flos-aquae, cyanobacterial toxins, LC-MS, saxitoxin