Analysis of Small-Subunit rDNA Sequences Obtained from Korean Peridinium bipes f. occultatum (Dinophyceae)

doi:2005.20.1.25

Algae > Volume 20(1); 2005 > Article

Algae 2005;20(1): 25-30. doi: https://doi.org/10.4490/algae.2005.20.1.025

Jang-Seu Ki, Soo-Yeon Cho and Myung-Soo Han^*

Department of Life Science, Hanyang University, Seoul 133-791, Korea

ABSTRACT

To clarify some confusions concerning identification of the Korean Peridinium species, genotypic analysis was performed with their SSU rDNA sequences. PCR was used to amplify the partial SSU rDNA of Peridinium isolates collected from three different Korean waters (Juam, Sang-sa and Togyo Reservoirs). The PCR products were allowed directly to sequence, which revealed each 942 bp of rDNA sequence. Analyses of the rDNA sequences showed that all the Korean isolates had the same genotype (100% sequence homology), and they were nearly identical to a Japanese strain of P. bipes f. occultatum (NIES 364; 99.8% sequence similarity). The sequence-based comparisons could clearly resolve P. bipes f. occultatum isolated from three different Korean waters.

Key words: Peridinium bipes, phylogenetic analysis, small-subunit rDNA