| The study on the Fluorescence Characteristics of Several Freshwater Bloom Forming Algal Species and Its Application
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| Moon-Ho Son, Ismayil S. Zulfugarov1, O-Seob Kwon, Byoung-Young Moon2, Ik Kyo Chung3, Choon-Hwan Lee1 and Jin Ae Lee*
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Department of Environmental Science and Engineering, Inje University, Kimhe 621-749,
1Department of Molecular Biology, Pusan National University, Busan 609-735,
2School of Biotechnology and Biomedical Science, Inje University, Kimhe 621-749 and
3Department of Marine Science, Pusan National University, Busan 609-735, Korea
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| ABSTRACT |
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The freshwater blooms mainly blue-green algal blooms occur frequently in the lower Naktong River in summer, which provoke many socio-economical problems; therefore, the early detection of bloom events are demanding through the quantitative and qualitative analyses of blue green algal species. The in vivo fluorescence properties of cultured strains of Microcystis aeruginosa, M. viridis, M. wesenbergii, M. ichthyoblabe, Anabaena cylindrica, A. flos-aquae, and Synedra sp. were investigated. Wild phytoplankton communities of the lower Naktong River were also monitored at four stations in terms of their standing stocks, biomass and fluorescence properties compared with its absorption spectram. The 77K fluorescence emission spectra of each cultured strains normalized at 620 nm was very specific and enabled to detect of blue green algal biomass qualitatively and quantitatively. The relative chlorophyll a concentration determined by chlorophyll fluorescence analysis method showed significant relationship with chlorophyll a concentration determined by solvent extraction method (R2 = 0.906), and the blue-green algal cell number determined by microscopic observation (R2 = 0.588), which gives insight into applications to early detection of blue green algal bloom.
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| Key words:
blue-green algal bloom, chlorophyll, fluorescence, Microcystis, 77K fluorescense |
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