Antibiotics and Their Optimum Concentration for Axenic Culture of Marine Microalgae

doi:2007.22.3.229

Algae > Volume 22(3); 2007 > Article

Algae 2007;22(3): 229-234. doi: https://doi.org/10.4490/algae.2007.22.3.229

Joo-Yeon Youn and Sung Bum Hur^*

Korea Marine Microalgae Culture Center, Department of Aquaculture, Pukyong National University, Nam-gu, Busan 608-737, Korea

ABSTRACT

This study was to determine the extent of bacteria contamination and resistance to various antibiotics used commonly in microalgal culture. Seven different dose levels of chloramphenicol, dihydrostreptomycin sulphate, neomycin, penicillin G, streptomycin sulphate, penicillin G + streptomycin sulphate, and penicillin G + streptomycin sulphate + chloramphenicol were added to each culture of microalgae. The lethal effects on microalgae and bacteria were the highest in chloramphenicol and the lowest in penicillin G. The axenic culture of bacillariophyceae and dinophyceae was more difficult than that of chlorophyceae and haptophyceae because of their complicate external morphology. The efficient antibiotics and their concentrations for axenic cultures varied with microalgal species. The optimum quantity for antibiotic treatments were 2,000 ppm of dihydrostreptomycin for Chlorella ellipsoidea, neomycin 500 ppm of Isochrysis galbana and Heterosigma ahashiwo, chloramphenicol 500 ppm of Cyclotella didymus, and dihydrostreptomycin sulphate and neomycin 6,000 ppm of Thalassiosira allenii.

Key words: antibiotics treatment, axenic culture, marine microalgae