Detection of Fish Killing Dinoflagellates Cochlodinium polykrikoides and Karlodinium veneficum (Dinophyceae) in the East China Sea by Real-time PCR

doi:2009.24.2.105

Algae > Volume 24(2); 2009 > Article

Algae 2009;24(2): 105-110. doi: https://doi.org/10.4490/algae.2009.24.2.105

Tae-Gyu Park^*, Yang-Soon Kang, Young-Tae Park, Heon-Meen Bae and Yoon Lee

Marine Ecology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea

*Corresponding Author

Email: taegyupark@gmail.com

ABSTRACT

The rDNAs of fish-killing dinoflagellates Cochlodinium polykrikoides and Karlodinium veneficum were detected from the East China Sea by species-specific real-time PCR probes. Sequence analyses using the partial ITS sequences from the real-time PCR products showed identical sequences with C. polykrikoides and K. veneficum, respectively and low expectation values (E-value) of less than 1e-5 suggesting the presence of these organisms in the East China Sea shelf water that flows into the Tsushima Strait and the Yellow Sea.

Key words: Cochlodinium polykrikoides, East China Sea, Karlodinium veneficum, real-time PCR, red tide