Culture study on the hybrid by interspecific crossing between Porphyra pseudolinearis and P. dentata (Bangiales, Rhodophyta), two dioecious species in culture

doi:2011.26.1.79

Algae > Volume 26(1); 2011 > Article

Algae 2011;26(1): 79-86. doi: https://doi.org/10.4490/algae.2011.26.1.079

Nam-Gil Kim

Department of Marine Biology and Aquaculture / Institute of Marine Industry, Gyeongsang National University, Tongyeong 650-160, Korea

*Corresponding Author

Email: ngkim@gnu.ac.kr

ABSTRACT

Physiological studies on the hybrid by crossing between two dioecious species, Porphyra pseudolinearis and P. dentata from Korea were conducted at constant temperatures (5, 10, 15, 20, and 25°C), at photon flux densities (10, 20, 40, and 80 μmol m-2 s-1) under photoperiods (14 L : 10 D and 10 L : 14 D). In the hybrid, higher growth of conchocelis was observed at 20 and 40 μmol m-2 s-1 under 14 L : 10 D. Conchosporangial branches were produced under 10-80 μmol m-2 s-1 at only 25°C, and were abundant when the conchocelis was cultured under 10 L : 14 D. Foliose thalli of the hybrid grew well at the conditions of 10-20°C, 10 L : 14 D and 15-20°C, 14 L : 10 D. The foliose thalli grew very slowly at 5°C and 30°C, respectively. No archeospores were observed at any culture conditions. Spermatangial and zygotosporangial sori were formed at the marginal portion of mature thallus. Zygotospores from the hybrid were released at 10-20°C under both photoperiods, and gave rise to form conchocelis filament. Monoecious thalli were observed at 10°C under 14 L : 10 D. Neither monospores nor protothalli were produced from the conchocelis in culture.

Key words: Bangiales; hybrid; interspecific crossing; physiology; Porphyra dentata; Porphyra pseudolinearis; Rhodophyta