| Home | E-Submission | Sitemap | Contact Us |  
top_img
Algae > Volume 27(4); 2012 > Article
Algae 2012;27(4): 295-301. doi: https://doi.org/10.4490/algae.2012.27.4.295
Influence of CO2 concentration on carbon concentrating mechanisms in cyanobacteria and green algae: a proteomic approach
Rishiram Ramanan, Nadimuthu Vinayagamoorthy, Saravana Devi Sivanesan, Krishnamurthi Kannan, Tapan Chakrabarti

1Environmental Health Division, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur 440 020, Maharashtra, India
*Corresponding Author  Email: k_krishnamurthi@neeri.res.in
ABSTRACT
Carbon concentrating mechanisms play a vital role in photosynthesis in microalgae and cyanobacteria especially in the proper functioning of Rubisco and assimilation of carbon via the Calvin cycle. This study evaluates the role of carbon dioxide on carbon concentrating mechanism (CCM) in a cynaobacteria, Spirulina platensis and a microalga, Chlorella sp. 786. The study organisms were grown in both atmospheric (control sample, 0.035%) and high (exposed sample, 10%) CO2 concentrations. Second dimension (2D) electrophoresis revealed a huge difference in the protein profiles of both organisms suggesting the induction of CCM related proteins in the sample maintained at atmospheric CO2 concentration and the repression of CCM related proteins in the sample maintained at 10% CO2. Liquid chromatography-mass spectroscopy analysis revealed the presence of two important Ci transporter proteins in the control sample of S. platensis, namely ferredoxin-NADP+ reductase and ATP binding cassette (ABC) transport system protein. These proteins were only expressed in the control sample and were downregulated or not expressed at all in the exposed sample. Consequently, this study conclusively proves that CCMs are only inducted at low CO2 concentrations and are not functional at high CO2 concentration.
Key words: carbon concentrating mechanism (CCM); Chlorella sp.; cyanobacteria; proteomics; Rubisco; Spirulina


TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Share:      
METRICS
12
Crossref
0
Scopus
4,168
View
131
Download
Related article
Editorial Office
[14348] A-1716, Gwangmyeong Trade Center, 72 Iljik-ro Gwangmyeong-si. Gyeonggi-do, Korea
Tel: +82-2-899-5980  Fax: +82-2-899-5922    E-mail: editalgae@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © The Korean Society of Phycology.                 Developed in M2PI