Chemiluminescence immunochromatographic analysis for the quantitative determination of algal toxins

doi:2013.28.3.289

Algae > Volume 28(3); 2013 > Article

Algae 2013;28(3): 289-296. doi: https://doi.org/10.4490/algae.2013.28.3.289

Dongjin Pyo¹^,* and Taehoon Kim¹

¹Department of Chemistry, Kangwon National University, Chuncheon 200-701, Korea

*Corresponding Author

Email: pyod@kangwon.ac.kr

ABSTRACT

For the quantitative detection of algal toxin, microcystin, a chemiluminescence immunochromatographic assay method was developed. The developed system consists of four parts, chemiluminescence assay strip (nitrocellulose membrane), horse radish peroxidase labeled microcystin monoclonal antibodies, chemiluminescence substrate (luminol and hydrogen peroxide), and luminometer. The performance of the chemiluminescence immunochromatographic assay system was compared with high performance liquid chromatography (HPLC) detection. The detection limit of chemiluminescence immunochromatographic assay system is several orders of magnitude lower than with HPLC. The chemiluminescence immunochromatography and HPLC results correlated very well with the correlation coefficient (r2) of 0.979.

Key words: algal toxin; chemiluminescence immunochromatography; HPLC; microcystin